Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
Title:
Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
Author:
Baumann, Claudia
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (556 seconds)
Series:
Biology
General Note:
Title from resource description page
Abstract:
Here, we present a protocol for specific siRNA-mediated mRNA depletion followed by immunofluorescence analysis to evaluate meiotic spindle assembly and organization in mouse oocytes. This protocol is suitable for in vitro depletion of transcripts and functional assessment of different spindle and/or MTOC-associated factors in oocytes.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/53586