Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation
Title:
Inducing Cre-lox Recombination in Mouse Cerebral Cortex Through In Utero Electroporation
Author:
Bland, Katherine M.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (629 seconds)
Series:
Neuroscience
General Note:
Title from resource description page
Abstract:
Cell-autonomous functions of genes in the brain can be studied by inducing loss or gain of function in sparse populations of cells. Here, we describe in utero electroporation to deliver Cre recombinase into sparse populations of developing cortical neurons with floxed genes to cause loss of function in vivo.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/56675