Sequential Photo-bleaching to Delineate Single Schwann Cells at the Neuromuscular Junction
Title:
Sequential Photo-bleaching to Delineate Single Schwann Cells at the Neuromuscular Junction
Author:
Brill, Monika S.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (672 seconds)
Series:
Neuroscience
General Note:
Title from resource description page
Abstract:
Visualizing individual cells in densely packed tissues, such as terminal Schwann cells (SCs) at neuromuscular junctions (NMJs), is challenging. "Sequential photo-bleaching" allows delineating single terminal SCs, for instance in the triangularis sterni muscle explant, a convenient nerve-muscle preparation, where sequential bleaching can be combined with time-lapse imaging and post-hoc immunostainings.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/4460