Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo
Title:
Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo
Author:
Burkel, Brian
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (624 seconds)
Series:
Bioengineering
General Note:
Title from resource description page
Abstract:
Collagen is a core component of the ECM, and provides essential cues for several cellular processes ranging from migration to differentiation and proliferation. Provided here is a protocol for embedding cells within 3D collagen hydrogels, and a more advanced technique for generating randomized or aligned collagen matrices using PDMS microchannels.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/53989