Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells
Title:
Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells
Author:
Chakraborty, Sayan
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (554 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
Here, we present the protocols for utilizing the insect cell and baculovirus protein expression system to produce large quantities of plant secreted proteins for protein crystallization. A baculovirus expression vector has been modified with either GP67 or insect hemolin signal peptide for plant protein secretion expression in insect cells.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/58283