How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells
Title:
How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells
Author:
Chen, Vanessa
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (663 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
Here, we present a protocol that involves genetically coupled spectrally distinct photoactivatable and fluorescent proteins. These fluorescent protein chimeras permit quantification of the PA-FP fraction that is photoactivated to be fluorescent, i.e., the photoactivation efficiency. The protocol reveals that different modes of photoactivation yield different photoactivation efficiencies.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/58588