SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells
Title:
SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells
Author:
Cheruiyot, Collins
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (624 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
Here, we describe a quantitative proteomics method using the technique of stable isotope labeling by amino acids in cell culture (SILAC) to analyze the effects of HIV-1 infection on host exosomal proteomes. This protocol can be easily adapted to cells under different stress or infection conditions.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/54799