RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse
Title:
RiboTag Immunoprecipitation in the Germ Cells of the Male Mouse
Author:
Chukrallah, Lauren G.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (600 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
Here, we describe the immunoprecipitation of ribosomes and associated RNA from select populations of adult male mouse germ cells using the RiboTag system. Strategic breeding and careful immunoprecipitation result in clean, reproducible results that inform on the germ cell translatome and provide insight into the mechanisms of mutant phenotypes.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/60927