Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System
Title:
Structural Information from Single-molecule FRET Experiments Using the Fast Nano-positioning System
Author:
Dörfler, Thilo
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (750 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
We present the setup and experimental procedure to obtain smFRET data from large donor-acceptor networks with a TIRF microscope. The step-by-step analysis of these measurements with the Bayesian inference software Fast-NPS yields high-resolved structural information via the application of adapted dye models.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/54782