Contents -- Preface -- List of Contributors -- Chapter 1 A Microscale Bioinspired Cochlear-like Sensor Robert D. White, Robert Littrell, and Karl Grosh -- Introduction -- Physiology and Design -- Microfabrication -- Experimental Results -- Mathematical Modeling -- Conclusions -- Acknowledgment -- References -- Chapter 2 Systematic Evaluation of the Efficiencies of Proteins and Chemicals in Pharmaceutical Applications Morgan Hamon and Jong Wook Hong -- Introduction -- Microbioreactors for Drug Target Selection -- Proteomics and genomics -- Metabolomics -- Microfluidic Devices for Compound Generation -- Lead Identification in Microfluidic Systems -- Protein crystallization -- Identification of "hit" compounds -- Droplet-based microfluidic devices for HTS -- Microdevices Useful in Lead Optimization Processes -- Dose-response analysis and IC50 measurement -- ADME/Tox evaluation -- Summary and Perspectives -- Acknowledgments -- References -- Chapter 3 Microfluidic Glucose Sensors Jithesh V. Veetil, Sruthi Ravindranathan, Sha Jin, and Kaiming Ye -- Introduction -- Classification of Microfluidic Glucose Sensors Based on their Fabrication Methods -- Lab-on-a-chip -- Lab-on-a-paper -- Lab-on-a-disk -- Enzyme-based Microfluidic Glucose Sensors -- Amperometric glucose sensors -- Enzyme-based optical microfluidic glucose sensors -- Enzymatic glucose sensors with integrated sampling systems -- Enzyme-free Glucose Detection Systems -- Microfluidic glucose sensors on carbon nanotubes -- Microfluidic glucose sensors on paper and foil -- Challenges and Prospectives -- References -- Chapter 4 Applications of Microfabrication and Microfluidic Techniques in Mesenchymal Stem Cell Research Abhijit Majumder, Jyotsna Dhawan, Oren Levy, and Jeffrey M. Karp -- Introduction -- Mesenchymal stem cells and their importance in regenerative medicine.

Challenges in MSC research and the role of microfabrication and microfluidics -- Microfabrication: Cell-adhesive islands and micro-/ nanotopography -- Micropatterning of cell-adhesive islands -- Effect of cell size -- Effect of cell shape -- Effect of substrate topology on MSC fate -- Importance of topography -- Role of microfabrication -- Artificially created topology: Structures and materials -- Structures -- Materials -- Micro-/nanogrooves -- Effect of pattern size on MSC cytoskeleton, morphology, and alignment -- Effect of pattern size on MSC differentiation -- Effect of pattern size on proliferation -- Micro-/nanopits: Effect of disorder -- A few other shapes: Effect of shape -- Substrates with micropillars -- Nanotubes and nanowires -- Application of Microfluidics -- Importance of flow in cell biology -- Advantages of microfluidics -- Problems of microfluidics -- Microfluidics in MSC research -- Conclusions and Outlook -- Suggested Reviews -- Stem cells and MSCs -- Microscale techniques and biology -- Mechanotransduction -- Micropatterning -- Effect of topology -- Microfluidics -- Acknowledgement -- References -- Chapter 5 Patient-Specific Modeling of Low-Density Lipoprotein Transport in Coronary Arteries Ufuk Olgac -- Introduction -- Methods -- Acquisition and processing of anatomy data -- Reconstruction of the flow field in the artery lumen -- Determination of blood plasma and LDL fluxes through the endothelium -- Reconstruction of flow and LDL concentration fields in the arterial wall -- Results -- Summary -- References -- Chapter 6 Point-of-Care Microdevices for Global Health Diagnostics of Infectious Diseases Sau Yin Chin, Tassaneewan Laksanasopin, Curtis D. Chin, and Samuel K. Sia -- Introduction -- Proteins: Immunoassays -- Fluid control -- Fluid actuation and delivery -- Signal detection -- Nucleic Acid-based Testing.

Sample preparation -- Nucleic acid amplification -- Product detection -- Cell-based Diagnostics -- Future Applications -- Companion diagnostics -- Proteomics -- Summary and Outlook -- References -- Chapter 7 Integrated Microfluidic Sample Preparation for Chip-based Molecular Diagnostics Jane Y. Zhang, Qingqing Cao, Madhumita Mahalanabis, and Catherine Klapperich -- Introduction to PCR -- History of PCR On-Chip -- Current Research in PCR On-Chip -- Sample Preparation for Standard PCR -- Tube methods for bacterial pathogens -- Tube methods for viral pathogens -- Variations on tube methods for sample preparation from human body fluids -- Sample Preparation Methods in Microfluidic Chips -- Brief Review of Current Chips Integrating Sample Preparation and PCR -- Major Challenges Going Forward -- References -- Chapter 8 Microfluidic Devices for Cellular Proteomic Studies Yihong Zhan and Chang Lu -- Introduction -- Trapped Cells for Time-Lapse Studies -- Cell Culture and Co-Culture -- Flow Cytometry -- Analysis after Lysis -- Other Analytical Techniques -- Conclusions -- References -- Chapter 9 Microfluidics for Neuroscience: Novel Tools and Future Implications Vivian M. Hernandez and P. Hande Özdinler -- Introduction -- Challenges in the field of neuroscience -- The brain is a complex system -- Neuronal polarity and heterogeneity -- Microfluidics as Novel Tools and Solutions to Limitations -- Applications of microfluidic devices in neuroscience -- Neuron type-specific analysis -- Axon biology -- Directed neuronal differentiation -- Introduction of neuron type-specific analysis to drug discovery -- Investigation of cellular interactions -- Summary -- Acknowledgments -- References -- Chapter 10 Microfluidics: On-Chip Platforms as In Vitro Disease Models Shan Gao, Erkin Seker, and Martin L. Yarmush -- Introduction.

Significance of in vitro platforms to study diseases -- Microfluidics for an in vitro disease model: Significance and advantages -- Microfluidic Cell Culture and Manipulation of the Microenvironment -- Controlling the cellular microenvironment with microfluidics -- Primary cell culture in microfluidics: Toward in vitro tissue models -- Complex culture systems -- Microfluidic Platforms for Bioanalytical Assays -- Molecular detection: Proteomic and metabolic analyses -- Genomic analyses -- Integrated Microfluidic Platforms to Study Diseases -- Microfluidic cell arrays -- Liver bioreactors -- Microfluidics in neurosciences -- Microfluidics in Vascular Research -- Conclusion and Outlook -- Acknowledgments -- References -- Chapter 11 Application of Microfluidics in Stem Cell and Tissue Engineering Sasha H. Bakhru, Christopher Highley, and Stefan Zappe -- Introduction -- Properties of Stem Cells -- Origin, properties, and isolation of stem cells -- In vivo adult stem cell niches -- Responses of stem cells to single environmental cues -- Microfluidics for Stem Cell Culture and Analysis -- Microfluidics for stem cell culture -- Microscale technologies for advanced stem cell culture -- Microfluidics for stem cell analysis -- Microfluidics for Tissue Engineering -- Hydrogels in microfluidic systems for three-dimensional tissue engineering -- Microfluidic strategies for efficient mass transport or vascularization -- Microfluidics for generation of modular tissue engineering constructs -- Microfluidics for the generation of tissue models -- Conclusion and Outlook -- References -- Chapter 12 Microfluidic "On-the-Fly" Fabrication of Microstructures for Biomedical Applications Edward Kang, Sau Fung Wong, and Sang-Hoon Lee -- Polymerizing Methods -- Photopolymerization -- Chemical polymerization -- Microfluidic platforms for shaped flow generation.

PDMS-based planar channels -- Capillary channels -- Fusion of PDMS-based planar and cylindrical channels -- Generated microstructures -- Spherical structures -- Fibrous and tubular structures -- Complicated structures and parallel production -- Biomedical applications -- Cell and drug encapsulation -- Tissue engineering -- Biosensing and assays -- Stimuli-responsive microactuators -- Conclusions -- References -- Chapter 13 Microfluidics as a Promising Tool Toward Distributed Viral Detection Elodie Sollier and Dino Di Carlo -- Introduction -- Viruses, Viral Samples, and Challenges of Viral Detection -- Viral particles and their components -- Samples classically used for viral detection -- Challenges of the sample matrix -- Macroscale Process -- Methods of concentration -- Methods of detection -- Direct detection of viral particles -- Detection of viral molecular fingerprints -- Detection of anti-viral antibodies -- Detection of viral antigens -- Detection of viral genomes -- Toward viruses-on-a-chip -- Viral Detection on Chip -- Molecular detection of viruses on chip -- Detection of anti-viral antibodies -- Detection of viral antigens -- Detection of viral genomes -- Direct detection of viruses on chip -- Microfluidic Methods of Virion Concentration -- Integrated ultrafiltration -- Microfluidics and functionalized beads -- Microfluidics and continuous virion separation -- From micro- to nanoscale bioparticles -- Conclusion -- References -- Chapter 14 Electrophoresis and Dielectrophoresis for Lab-on-a-Chip (LOC) Analyses Yagmur Demircan, Gürkan Yilmaz, and Haluk Külah -- Introduction -- Electrophoresis -- Theory of electrophoresis -- Electrophoretic force -- Double-layer generation -- Viscous drag force and electroosmosis -- Applications of electrophoresis -- DNA electrophoresis -- Mutation detection -- Protein electrophoresis -- Conclusion.

Dielectrophoresis.