Use of Drosophila S2 Cells for Live Imaging of Cell Division
Title:
Use of Drosophila S2 Cells for Live Imaging of Cell Division
Author:
Dewey, Evan B.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (6 seconds)
Series:
Genetics
General Note:
Title from resource description page
Abstract:
Cell divisions can be visualized in real time using fluorescently tagged proteins and time-lapse microscopy. Using the protocol presented here, users can analyze cell division timing dynamics, mitotic spindle assembly, and chromosome congression and segregation. Defects in these events following RNA interference (RNAi)-mediated gene knockdown can be assessed and quantified.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/60049