Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach
Title:
Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach
Author:
Dhoondia, Zuzer
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (732 seconds)
Series:
Genetics
General Note:
Title from resource description page
Abstract:
We describe a basic experimental approach for analysis of termination of transcription by RNA polymerase II in vivo using BrUTP by the strand-specific transcription run-on (TRO) approach in budding yeast. This protocol can be extended to study transcription termination by other RNA polymerases both in yeast and higher eukaryotes.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/55446