Spatial and Temporal Analysis of Active ERK in the C. elegans Germline
Title:
Spatial and Temporal Analysis of Active ERK in the C. elegans Germline
Author:
Gervaise, Amanda L.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (520 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
We present an immunofluorescence imaging-based method for spatial and temporal localization of active ERK in the dissected C. elegans gonad. The protocol described here can be adapted for visualization of any signaling or structural protein in the C. elegans gonad, provided a suitable antibody reagent is available.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/54901