Generation of Fluorescent Protein Fusions in Candida Species
Title:
Generation of Fluorescent Protein Fusions in Candida Species
Author:
Gonia, Sara
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (567 seconds)
Series:
Genetics
General Note:
Title from resource description page
Abstract:
PCR-mediated gene modification can be used to generate fluorescent protein fusions in Candida species, which facilitates visualization and quantitation of yeast cells and proteins. Herein, we present a strategy for constructing a fluorescent protein fusion (Eno1-FP) in Candida parapsilosis.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/55333