Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
Title:
Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
Author:
Hiener, Bonnie
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (618 seconds)
Series:
Genetics
General Note:
Title from resource description page
Abstract:
Full-length individual proviral sequencing (FLIPS) provides an efficient and high-throughput method for the amplification and sequencing of single, near full-length (intact and defective) HIV-1 proviruses and allows for determination of their potential replication-competency. FLIPS overcomes limitations of previous assays designed to sequence the latent HIV-1 reservoir.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/58016