siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts
Title:
siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts
Author:
Lokossou, Adjimon Gatien
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (597 seconds)
Series:
Genetics
General Note:
Title from resource description page
Abstract:
This protocol describes a method for efficiently transfecting siRNA in freshly isolated human villous cytotrophoblasts using microporation and identifying DNA-protein complexes in these cells. Transfected cells can be monitored by Western blot and EMSA analyses during the 4-day culture time.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/53995