Adapting 3' Rapid Amplification of CDNA Ends to Map Transcripts in Cancer
Title:
Adapting 3' Rapid Amplification of CDNA Ends to Map Transcripts in Cancer
Author:
Masamha, Chioniso Patience
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (578 seconds)
Series:
Genetics
General Note:
Title from resource description page
Abstract:
The two different 3' rapid amplification of cDNA ends (3' RACE) protocols described here make use of two different DNA polymerases to map sequences that include a segment of the open reading frame (ORF), the stop codon, and the entire 3' UTR of a transcript using RNA obtained from different cancer cell lines.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/57318