Development of a Negative Selectable Marker for Entamoeba histolytica
Title:
Development of a Negative Selectable Marker for Entamoeba histolytica
Author:
Abhyankar, Mayuresh M
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (365 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
We report development of a negative selection system in E. histolytica based upon transgenic expression of a chimeric protein (FCU1) and selection with the prodrug 5-fluorocytosine. The FCU1 protein is a fusion of yeast cytosine deaminase and uracil phosphoribosyltransferase. Expression of FCU1 resulted in increased E. histolytica sensitivity towards 5-fluorocytosine.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/2410