Preparation of a High-quality Primary Cell Culture from Fish Pituitaries
Title:
Preparation of a High-quality Primary Cell Culture from Fish Pituitaries
Author:
Ager-Wick, Eirill
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (456 seconds)
Series:
Neuroscience
General Note:
Title from resource description page
Abstract:
Here we describe a protocol to prepare and maintain primary pituitary cell cultures from medaka (Oryzias latipes). The optimized conditions in this protocol take important parameters such as temperature, osmolality, and pH into consideration by mimicking the physiological conditions of the fish, thereby enabling physiologically more meaningful results.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/58159