Generation of Organotypic Raft Cultures from Primary Human Keratinocytes
Title:
Generation of Organotypic Raft Cultures from Primary Human Keratinocytes
Author:
Anacker, Daniel
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (446 seconds)
Series:
Immunology and Infection
General Note:
Title from resource description page
Abstract:
An in vitro method to mimic in vivo epithelial differentiation is described. Many viruses target epithelial cells as part of their viral life cycle, and this method provides a means of examining virus:host interactions that more closely resembles that which occurs in vivo. This technique can be used with primary keratinocytes, established cell lines, as well as normal or diseased biopsy tissue.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/3668