Title Page -- Copyright Page -- Contents -- About the Editors -- Contributors -- Preface -- Part 1 An Introduction to Stem Cells -- Chapter 1 Adult Stem Cells -- Introduction -- Adult Stem Cells -- Adult Stem-Cell Plasticity -- Classification of Adult Stem Cells -- Hematopoietic Stem Cells -- Mesenchymal Stem Cells -- Aging -- Aging of ASCs and Possible Consequences -- Stem Cells in Regenerative Medicine and in vitro Application -- Summary -- Acknowledgments -- References -- Chapter 2 Pluripotent Stem Cells -- Introduction -- Induced Pluripotent Stem Cells -- Pluripotent Stem Cells from Germ Cells of the Testis -- Regulation of PSCs -- Application of Nanotechnology to PSCs -- Conclusions -- References -- Chapter 3 Interactions of Stem Cells and Components of the Extracellular Matrix -- Introduction -- Chemical Properties of the ECM -- Topographical Features of the ECM -- Mechanical Properties of ECM -- Perspective -- References -- Chapter 4 Regenerative Medicine and Cell Therapy: Past, Present, and Future -- Introduction -- Basics of Regenerative Medicine -- Cell Types Used in Regenerative Medicine -- Extracellular Matrix In Vivo: Components and Behavior of Cells on Them -- Regenerative Medicine and Cell Therapy: Past, Present, and Future -- Summary and Conclusion -- Acknowledgment -- References -- Part 2 An Introduction to Nanotechnology -- Chapter 5 Principles of Nanotechnology -- Introduction -- General Characteristics of Nanomaterials -- Nanotechnology -- Classification of Nanomaterials -- Environmental and Health Effects of Nanomaterials -- Conclusions -- Acknowledgments -- References -- Chapter 6 Stem-Cell Nanoengineering: Explorations in a Rapidly Moving Field -- Introduction -- Need for Stem-Cell Nanoengineering -- Nanoengineering to Study Stem-Cell Niche -- Nanoengineering to Study Stem-Cell Homing.

Nanoscale Approaches to Elucidate Stem-Cell and ECM Interaction -- Nanoengineering Approaches to Study Stem-Cell Fate -- Conclusions and Future Prospective -- Acknowledgments -- References -- Part 3 Nanostructures for Stem-Cell Engineering - Engineering Approach -- Chapter 7 Nanopatterned Surfaces for Stem-Cell Engineering -- Introduction -- Technology for Nanopatterned Surface of Cell Chips -- Application of Nanopatterned Surface to Stem-Cell-Based Chips -- Conclusions, Challenges, and Future Perspectives -- Acknowledgments -- References -- Chapter 8 Biomimetic Nanostructures by Electrospinning and Electrospraying -- Introduction -- Biomimetic Nanofibrous Scaffolds -- Nanofiber Fabrication Techniques -- The Effect of Nanofibrous Scaffold Parameters on Stem-Cell Behavior -- Regulation of Electrospun Nanofibers -- Bioelectrospraying and Cell-Electrospinning -- Conclusions -- Acknowledgements -- References -- Chapter 9 Nanoparticles for Stem-Cell Engineering -- Introduction -- Nanoparticle Toxicity to Stem Cells -- Nanoparticles for Detecting Stem Cells -- Nanoparticles for Growth Factor Delivery to Stem Cells -- Nanoparticles for Gene Delivery to Stem Cells -- Nanoparticles for Patterning Stem Cells -- Conclusion -- Acknowledgments -- References -- Chapter 10 Toxicology of Nanobiomaterials -- Introduction -- Cytotoxicity Assessment -- Genotoxicity of Nanomaterials -- Immunotoxicity of Nanomaterials -- Carcinogenicity and Reproductive Toxicity -- Blood Compatibility of Nanomaterials -- Conclusions -- References -- Part 4 Control of Stem-Cell Fate by Engineering of Microenvironment -- Chapter 11 Stem-Cell Responses to Surface Nanotopographies -- Introduction -- Responses of Stem Cells to Nanoprotrusion Surfaces -- Responses of Stem Cells to Nanoporous Substrates -- Responses of Stem Cells to Nanogrooved Surfaces.

Responses of Stem Cells to Nanofibrous Structures -- The Mechanism Governing Nanotopographical Control of Stem Cell Fate -- Conclusions -- References -- Chapter 12 Control of Mesenchymal Stem-Cell Fate by Engineering the Nanoenvironment -- Introduction -- Bionanotechnology and Regenerative Medicine -- Mesenchymal Stem Cells -- Cell Structure -- Cells and Mechanical Forces -- Mechanotransduction -- Focal Adhesion Complexes -- Linking the Cytoskeleton to the Nucleus and Direct Mechanotransduction -- Direct Stimulation of Nanomechanotransduction "Nanokicking" -- Bone Bioreactors -- Conclusions and Future Outlooks -- Acknowledgments -- References -- Chapter 13 Delivery of Molecules and Genes/Small Interfering RNA into Stem Cells by Nanoengineering -- Introduction -- Delivered Bioactive Agents into Stem Cells -- Nanoengineered Carriers for Intracellular Delivery into Stem Cells -- Conclusions -- Acknowledgments -- References -- Part 5 Nanotissue Engineering - Biological Approach along with Differentiation -- Chapter 14 Expansion of Stem Cells by Nanotissue Engineering -- Introduction -- Design Principles of Nanostructured Biomaterials -- Nanofibers -- Nanocomposites -- Surface-Modified Nanostructures -- Conclusions -- References -- Chapter 15 Nanotissue Engineering of Neural Cells -- Introduction -- The Role of ECM Components/Engineering ECM Signals into Biomaterials -- Role of Growth Factors, Signals, and Bioactive Molecules -- Effects of Physico-Mechanical Cues -- Final Remarks -- References -- Chapter 16 Nanotechnology and Cardiovascular Tissue Engineering -- Introduction -- Cell Therapies in Cardiovascular Diseases -- Cardiac Tissue Engineering -- Vascular Tissue Engineering -- Nanotechnology and Cardiovascular Tissue Engineering -- Conclusions -- References -- Chapter 17 Nanotissue Engineering of Musculoskeletal Cells -- Introduction.

Nanostructures in Normal Musculoskeletal Tissues -- Stem Cells for Musculoskeletal Tissue Nanoengineering -- Cell-Nanomaterial Interaction -- Nanotissue Engineering for Musculoskeletal Tissue -- Challenges -- Conclusions and Future Outlook -- References -- Chapter 18 Nanotissue Engineering of Skin Cells -- Introduction -- Skin and Skin Loss -- Adult Stem Cells for Skin Regeneration -- Extracellular Matrix -- Engineered ECMs -- Nanotopography -- Electrospinning -- Natural Polymers -- Synthetic Polymer Blends -- Bioactive Nanofibers -- Conclusion and Future Outlooks -- Acknowledgment -- References -- Chapter 19 High-Throughput Screening of Stem Cell Self-Renewal and Differentiation on Nanomaterials -- Introduction -- Electrical Cell-Substrate Impedance -- Microfluidic Flow Cytometry -- Electrochemical Methods -- Raman Scattering-Based Methods -- Microscopy-Based Methods -- Conclusions -- Acknowledgments -- References -- Part 6 Nanotechnology in Stem-Cell Imaging -- Chapter 20 Nanotechnology for Cellular Imaging -- Introduction -- The Need for Cellular Imaging in a Clinical Setting -- The Ultimate Goals of Stem-Cell Imaging -- Nanotechnology for Stem-Cell Imaging -- Iron Oxide Nanoparticles -- Gadolinium Nanoparticles -- Manganese Oxide Nanoparticles -- Fluorine Nanoparticles -- PARACEST Nanoparticles -- Gold and Tantalum Nanoparticles -- Polymer Dots -- Nanodiamonds -- Upconverting Luminescence Nanoparticles -- Nanobubbles -- Multimodal Nanoparticles -- Summary -- Acknowledgments -- References -- Part 7 Nanotissue Engineering and Clinical Applications -- Chapter 21 Advancing Translational Nanotechnology to Clinical Application -- Introduction -- Nanomaterials for Organ Regeneration -- The Ethics of Nanotechnology in Regenerative Medicine -- Translating Science to Product -- References -- Chapter 22 Stem-Cell Nanoengineering from Bench to Bed.

Introduction -- Legislative Authority -- The FDA Regulations: Human Tissue for Transplantation -- Ethical Issues -- Public Information Websites Related to Tissue-Engineered Products -- Nanoengineering in Clinical Application -- Nanocarriers -- Nanostructure Role in Clinical Tissue Regeneration -- Nanotopographies -- Smart Substrates -- Conclusions -- References -- Index -- Supplemental Images -- EULA.