Chromatography And Capillary Electrophoresis In Food Analysis -- Contents -- Chapter 1 General Aspects of Experimental Biochemistry -- 1 Introduction -- 2 Quantities, Symbols, and Units -- 3 Basic Mathematics and Statistics -- 4 Laboratory Safety -- 5 Recording and Reporting Laboratory Results -- 6 Selected General and Specific Literature -- Chapter 2 Buffers and Micelles -- 1 Introduction -- 2 Protolytic Activity, Acids, and Bases -- 3 Activity Coefficients and Ionic Strength -- 4 Electrolytes and Ampholytes -- 5 Buffer Solutions and Buffer Capacity -- 6 Preparation of Buffers -- 7 Selection of Buffers -- 8 Organic Solvents as Modifiers of Aqueous Solutions -- 9 Amphipathic Molecules and Micelles in Aqueous Solution -- 10 Selected General and Specific Literature -- Chapter 3 Binding, Association, Dissociation, and Kinetics -- 1 Introduction -- 2 Binding Forces - Position of Equilibrium -- 3 Specificity of Proteins/Enzymes Towards Ligands and Antinutrients -- 4 Analysis of Binding Experiments -- 5 Selected General and Specific Literature -- Chapter 4 Extraction of Native Low-Mr and High-Mr Biomolecules -- 1 Introduction -- 2 Extraction of Low-Mr Biomolecules -- 3 Amphipathic Membrane Constituents -- 4 Dialysis -- 5 Ultrafiltration -- 6 Centrifugation -- 7 Selected General and Specific Literature -- Chapter 5 Spectroscopy and Detection Methods -- 1 Introduction -- 2 Atomic Spectroscopy -- 3 UV-Vis Spectroscopy -- 4 Colorimetry -- 5 Fluorimetry -- 6 Diode-array Detection -- 7 Infrared Spectroscopy (IR) -- 8 Nuclear Magnetic Resonance (NMR) -- 9 Mass Spectrometry -- 10 Selected General and Specific Literature -- Chapter 6 Liquid Chromatography -- 1 Introduction -- 2 Separation of Analytes between the Two Phases in LC -- 3 Surface Tension, Osmotic Pressure, Viscosity, Frictional Coefficient, and Diffusion -- 4 Basic Concepts in Liquid Chromatography.

5 Paper and Thin-layer Chromatography -- 6 Permeation- or Size-exclusion Chromatography -- 7 Affinity Chromatography -- 8 Hydrophobic Interaction Chromatography -- 9 Solid Phase Extractions and Reversed Phase Chromatography -- 10 Selected General and Specific Literature -- Chapter 7 Ion-exchange Chromatography -- 1 Introduction -- 2 Ion-exchanger Materials -- 3 Choice of Ion-exchanger -- 4 Regeneration of Ion-exchangers -- 5 Packing of Ion-exchange Columns and Sample Application -- 6 Group Separation of Low-Mr Compounds -- 7 Column Chromatographic Separation of Low-Mr Compounds -- 8 Column Chromatographic Separation of High-Mr Compounds -- 9 Flash Chromatography -- 10 Chromatofocusing -- 11 Selected General and Specific Literature -- Chapter 8 High-performance Liquid Chromatography and Fast Polymer Liquid Chromatography -- 1 Introduction -- 2 Instrumentation -- 3 Columns and Column Materials -- 4 Liquid Chromatography Principles including Ion-pair Reversed-phase Chromatography and Chiral Chromatography -- 5 Applications of HPLC/FPLC -- 6 Selected General and Specific Literature -- Chapter 9 Electrophoresis -- 1 Introduction -- 2 Electro-osmosis, Stern Layer, and Zeta Potential -- 3 Zone Electrophoresis -- 4 Moving Boundary Electrophoresis - Isotachophoresis -- 5 Specialized Electrophoretic Techniques -- 6 Detection and Recovery -- 7 Selected General and Specific Literature -- Chapter 10 High-performance Capillary Electrophoresis -- 1 Introduction -- 2 Basic Principles -- 3 Instrumentation -- 4 Selected Modes of HPCE Operation and Examples of Applications -- 5 Parameters of Analysis Affecting Separation in HPCE -- 6 Development of a HPCE Method of Analysis -- 7 Conclusion -- 8 Selected General and Specific Literature -- Chapter 11 Analytical Determination of Low-Mr Compounds -- 1 Introduction -- 2 Inorganic Ions and Elemental Analyses.

3 Low-Mr Organic Compounds with Positive Net Charge, Alkaloids and Biogenic Amines -- 4 Ampholytes, Amino Acids, Peptides, and Heteroaromatics -- 5 Carboxylates, Phosphates, Sulfonates, and Sulfates3 -- 6 Low-Mr Compounds without Protolytically Active Groups. Carbohydrates, Glycosides, Esters -- 7 Special Groups of Bioactive Compounds -- 8 Conclusion -- 9 Selected General and Specific Literature -- Chapter 12 Protein Purification and Analysis -- 1 Introduction -- 2 Proteins in Food Matrices -- 3 Handling of Protein Fractions -- 4 Strategy for Protein Purification -- 5 Sample Preparation -- 6 Group Separation -- 7 High Resolution Techniques -- 8 Methods of Protein Analyses -- 9 Selected Examples of Protein Purification Procedures -- 10 Selected General and Specific Literature -- Chapter 13 Immunochemical Techniques -- 1 Introduction -- 2 Antibodies -- 3 Immunochemical Methods of Analyses -- 4 Immunoaffinity Chromatography -- 5 Selected General and Specific Literature -- Chapter 14 Analysis of Dietary Fibre -- 1 Introduction -- 2 Composition of DF -- 3 Gravimetric Methods of Analyses -- 4 Chromatographic Methods of Analyses -- 5 Further Characterization of DF -- 6 Conclusion -- 7 Selected General and Specific Literature -- Appendix Supercritical Fluid Extraction (SFE) and Supercritical Fluid Chromatography (SFC) -- 1 Introduction -- 2 Theory -- 3 SFE -- 4 SFC -- 5 Selected General and Specific Literature -- Subject Index.