Pooled CRISPR-Based Genetic Screens in Mammalian Cells
Title:
Pooled CRISPR-Based Genetic Screens in Mammalian Cells
Author:
Chan, Katherine
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (9 seconds)
Series:
Genetics
General Note:
Title from resource description page
Abstract:
CRISPR-Cas9 technology provides an efficient method to precisely edit the mammalian genome in any cell type and represents a novel means to perform genome-wide genetic screens. A detailed protocol discussing the steps required for the successful performance of pooled genome-wide CRISPR-Cas9 screens is provided here.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/59780