Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay
Title:
Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay
Author:
Chang, Ju-Chun
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (672 seconds)
Series:
Genetics
General Note:
Title from resource description page
Abstract:
This protocol describes a heat shock-induced protein expression system (pDHsp/V5-His/sf9 cell system), which can be used for either expressing foreign proteins or evaluating the anti-apoptotic activity of potential foreign proteins and their truncated amino acids in insect cells.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/56693