Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture
Title:
Evaluating the Differentiation Capacity of Mouse Prostate Epithelial Cells Using Organoid Culture
Author:
Crowell, Preston D.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (638 seconds)
Series:
Cancer Research
General Note:
Title from resource description page
Abstract:
Mouse prostate organoids represent a promising context to evaluate mechanisms that regulate differentiation. This paper describes an improved approach to establish prostate organoids, and introduces methods to (1) collect protein lysate from organoids, and (2) fix and stain organoids for whole-mount confocal microscopy.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/60223