LC-MS/MS in proteomics : methods and applications
Title:
LC-MS/MS in proteomics : methods and applications
Author:
Cutillas, Pedro R.
ISBN:
9781607617792
9781607617808
Publication Information:
New York, NY : Humana Press, c2010.
Physical Description:
x, 357 p. : ill. ; 27 cm.
Series:
Springer protocols
Methods in molecular biology, 658
Springer protocols.
Methods in molecular biology (Clifton, N.J.) ; v. 658.
Contents:
Approaches and applications of quantitative LC-MS for proteomics and activitomics / Pedro R. Cutillas and John F. Timms -- Overview of quantitative LC-MS techniques for proteomics and activitomics / John F. Timms and Pedro R. Cutillas -- Instrumentation for LC-MS/MS in proteomics / Robert Chalkley -- Bioinformatics for LC-MS/MS-based proteomics / Richard J. Jacob -- Analysis of post-translational modifications by LC-MS/MS / Hannah Johnson and Claire E. Eyers -- In-depth analysis of protein phosphorylation by multidimensional ion exchange chromatography and mass spectrometry / Maria P. Alcolea and Pedro R. Cutillas -- Mapping of phosphorylation sites by LC-MS/MS / Bertran Gerrits and Bernd Bodenmiller -- Analysis of carbohydrates on proteins by offline normal-phase liquid chromatography MALDI-TOF-MS/MS / Theodora Tryfona and Elaine Stephens -- Selected reaction monitoring applied to quantitative proteomics / Reiko Kiyonami and Bruno Domon -- Basic design of MRM assays for peptide quantification / Andrew James and Claus Jorgensen -- Proteome-wide quantitation by SILAC / Kristoffer T.G. Rigbolt and Blagoy Blagoev -- Quantification of proteins by iTRAQ / Richard D. Unwin -- Quantification of proteins by label-free LC-MS/MS / Yishai Levin and Sabine Bahn -- Protocol for quantitative proteomics of cellular membranes and membrane rafts / Andrew J. Thompson and Ritchie Williamson -- Organelle proteomics by label-free and SILAC-based protein correlation profiling / Joern Dengjel, Lis Jakobsen, and Jens S. Andersen -- Mapping protein-protein interactions by quantitative proteomics / Joern Dengjel, Irina Kratchmarova, and Blagoy Blagoev -- Analysis of serum proteins by LC-MS/MS / Sarah Tonack, John P. Neoptolemos, and Eithne Costello -- Urinary proteome profiling using 2D-DIGE and LC-MS/MS / Mark E. Weeks -- Analysis of peptides in biological fluids by LC-MS/MS / Pedro R. Cutillas -- Quantification of protein kinase activities by LC-MS / Maria P. Alcolea and Pedro R. Cutillas -- A protocol for top-down proteomics using HPLC and ETD/PTR-MS / Sarah R. Hart.