Detection of Protease Activity by Fluorescent Peptide Zymography
Title:
Detection of Protease Activity by Fluorescent Peptide Zymography
Author:
Deshmukh, Ameya A.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (596 seconds)
Series:
Cancer Research
General Note:
Title from resource description page
Abstract:
Here, we present a detailed protocol for a modified zymographic technique in which fluorescent peptides are used as the degradable substrate in place of native proteins. Electrophoresis of biological samples in fluorescent peptide zymograms enables detection of a wider range of proteases than previous zymographic techniques.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/58938