Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish
Title:
Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish
Author:
Dobrzycki, Tomasz
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (341 seconds)
Series:
Developmental Biology
General Note:
Title from resource description page
Abstract:
Gene editing technologies have enabled researchers to generate zebrafish mutants to investigate gene function with relative ease. Here, we provide a guide to perform parallel embryo genotyping and quantification of in situ hybridization signals in zebrafish. This unbiased approach provides greater accuracy in phenotypical analyses based on in situ hybridization.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/59956