Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient
Title:
Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient
Author:
Feltwell, Theresa
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (352 seconds)
Series:
Genetics
General Note:
Title from resource description page
Abstract:
We demonstrate the use of discontinuous density gradients to separate bacterial populations based on capsule production. This method is used to compare capsule amount between cultures, isolate mutants with a specific capsule phenotype, or to identify capsule regulators. Described here is the optimization and running of the assay.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/58679