Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation
Title:
Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation
Author:
Finney, Lydia A.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (474 seconds)
Series:
Chemistry
General Note:
Title from resource description page
Abstract:
Here, we present a protocol on how to determine the quantity and distribution of metals in a sample using synchrotron X-ray fluorescence. We focus on adherent cells, and describe the chemical fixation method to prepare this sample. We then describe how to mount and image the sample using synchrotron X-rays.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/52370