Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens
Title:
Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens
Author:
Foo, Alexander C. Y.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (549 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
This protocol describes the removal of endogenous lipids from allergens, and their replacement with user-specified ligands through reverse-phase HPLC coupled with thermal annealing. 31P-NMR and circular dichroism allow for the rapid confirmation of ligand removal/loading, and the recovery of native allergen structure.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/61780