A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
Title:
A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
Author:
Fu, Qin
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (432 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
Here, we present an automated plasma protein digestion method for mass spectrometry-based quantitative proteomic analysis. In this protocol, the liquid transferring and incubation steps for protein denaturation, reduction, alkylation, and trypsin digestion reactions are streamlined and automated. It takes approximately five hours to prepare a 96-well plate with desired precision.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/59842