Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
Title:
Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
Author:
Götz, Markus
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (682 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
Here, we present a protocol to obtain three-color smFRET data and its analysis with a 3D ensemble Hidden Markov Model. With this approach, scientists can extract kinetic information from complex protein systems, including cooperativity or correlated interactions.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/56896