Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders
Title:
Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders
Author:
Gall, Joseph G.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (607 seconds)
Series:
Genetics
General Note:
Title from resource description page
Abstract:
We present simple techniques for isolating giant transcriptionally active lampbrush chromosomes from living oocytes of frogs and salamanders. We describe how to observe these chromosomes "alive" by phase contrast or differential interference contrast, and how to fix them for fluorescent in situ hybridization or immunofluorescent staining.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/54103