Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy
Title:
Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy
Author:
Gemeinhardt, André
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (655 seconds)
Series:
Engineering
General Note:
Title from resource description page
Abstract:
We present a protocol for the real-time optical detection of single unlabeled proteins as they are secreted from living cells. This is based on interferometric scattering (iSCAT) microscopy, which can be applied to a variety of different biological systems and configurations.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/58486