A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells
Title:
A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells
Author:
Giolito, Maria Virginia
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (398 seconds)
Series:
Cancer Research
General Note:
Title from resource description page
Abstract:
This protocol presents a novel, robust, and reproducible culture system to generate and grow three-dimensional spheroids from Caco2 colon adenocarcinoma cells. The results provide the first proof-of-concept for the appropriateness of this approach to study cancer stem cell biology, including the response to chemotherapy.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/61783