Cover -- Contents -- Preface -- About the Authors -- SECTION I: The Biology behind the Technology -- 1 Fundamental Technologies -- Molecular Cloning -- Genomic Libraries -- Amplification of DNA Using PCR -- DNA Sequencing Technologies -- Sequencing Whole Genomes -- Genomics -- SUMMARY -- REVIEW QUESTIONS -- REFERENCES -- 2 Fundamental Concepts in Immunology -- The Immune Response -- Functions of the Immune System -- Cells of the Immune System: Cell-Mediated Immunity -- Tissues of the Immune System -- Humoral Immunity -- Types of Antibodies: Applications -- Immunological Techniques -- SUMMARY -- REVIEW QUESTIONS -- REFERENCES -- 3 The Genetic Basis of Disease -- Chromosomal Disorders and Gene Mapping -- Single-Gene Disorders -- Polygenic Disorders and Gene Clustering -- Mitochondrial Disorders -- SUMMARY -- REVIEW QUESTIONS -- REFERENCES -- 4 Immune Pathogenesis -- Models of Immune System Lesions -- Inflammation and Immune Hypersensitivity Disorders -- Immunodeficiency Disorders and Defects in Development of the Immune System -- SUMMARY -- REVIEW QUESTIONS -- REFERENCES -- 5 Microbial Pathogenesis -- Introduction -- Bacterial Infections -- Viral Infections -- SUMMARY -- REVIEW QUESTIONS -- REFERENCES -- SECTION II: Production of Therapeutic Agents -- 6 Modulation of Gene Expression -- Manipulating Gene Expression in Prokaryotes -- Heterologous Protein Production in Eukaryotic Cells -- Directed Mutagenesis -- SUMMARY -- REVIEW QUESTIONS -- REFERENCES -- 7 Genetic Engineering of Plants -- Plant Transformation with the Ti Plasmid of A. tumefaciens -- Physical Transfer of Genes to Plants -- Chloroplast Engineering -- Transient Gene Expression -- Molecular Pharming -- Edible Vaccines -- SUMMARY -- REVIEW QUESTIONS -- REFERENCES -- SECTION III: Diagnosing and Treating Human Disease -- 8 Molecular Diagnostics.

Immunological Approaches To Detect Protein Biomarkers of Disease -- DNA-Based Approaches to Disease Diagnosis -- Detecting RNA Signatures of Disease -- SUMMARY -- REVIEW QUESTIONS -- REFERENCES -- 9 Protein Therapeutics -- Pharmaceuticals -- Recombinant Antibodies -- Enzymes -- Lactic Acid Bacteria -- SUMMARY -- REVIEW QUESTIONS -- REFERENCES -- 10 Nucleic Acid Therapeutic Agents and Human Gene Therapy -- Treating Genetic and Nongenetic Disorders -- Targeting Specific mRNAs and DNAs -- Viral Delivery Systems -- Nonviral Nucleic Acid Delivery Systems -- Gene Therapy -- SUMMARY -- REVIEW QUESTIONS -- REFERENCES -- 11 Vaccines -- Vaccination: Overview -- Subunit Vaccines -- Peptide Vaccines -- Dendritic Cell Vaccines -- DNA Vaccines -- Attenuated Vaccines -- Vector Vaccines -- Adjuvants -- Systems Biology and Evaluation of Vaccines -- SUMMARY -- REVIEW QUESTIONS -- REFERENCES -- 12 Societal Issues -- Safety and Ethical Issues -- Patenting Biotechnology -- Economic Issues -- SUMMARY -- REVIEW QUESTIONS -- REFERENCES -- Glossary -- A -- B -- C -- D -- E -- F -- G -- H -- I -- K -- L -- M -- N -- O -- P -- Q -- R -- S -- T -- U -- V -- W -- X -- Y -- Index -- A -- B -- C -- D -- E -- F -- G -- H -- I -- J -- K -- L -- M -- N -- O -- P -- Q -- R -- S -- T -- U -- V -- W -- X -- Y -- Z.