Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection
Title:
Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection
Author:
Gonzalez, Walter G.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (602 seconds)
Series:
Chemistry
General Note:
Title from resource description page
Abstract:
Here we report an application of the photothermal beam deflection technique in combination with a caged calcium compound, DM-nitrophen, to monitor microsecond and millisecond dynamics and energetics of structural changes associated with the calcium association to a neuronal calcium sensor, Downstream Regulatory Element Antagonist Modulator.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/50969