Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells
Title:
Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells
Author:
Gordon, Shellaina J.V.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (784 seconds)
Series:
Biochemistry
General Note:
Title from resource description page
Abstract:
Cultured primary or established cell lines are commonly used to address fundamental biological and mechanistic questions as an initial approach before using animal models. This protocol describes how to prepare whole cell extracts and subcellular fractions for studies of zinc (Zn) and other trace elements with atomic absorbance spectroscopy.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/59519