Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells
Title:
Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells
Author:
Guillen-Ahlers, Hector
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (567 seconds)
Series:
Genetics
General Note:
Title from resource description page
Abstract:
This is a method to identify novel DNA-interacting proteins at specific target loci, relying on sequence-specific capture of crosslinked chromatin for subsequent proteomic analyses. No prior knowledge about potential binding proteins, nor cell modifications are required. Initially developed for yeast, the technology has now been adapted for mammalian cells.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/57140