The new era of biology in singulo / T. Ha and P.R. Selvin -- Single-molecule FRET with total internal reflection microscopy / C. Joo and T. Ha -- In vitro and in vivo FIONA and other acronyms for watching molecular motors walk / P.R. Selvin ... [et al.] -- Colocalization of fluorescent probes : Accurate and precise registration with nanometer resolution / L.S. Churchman and J.A. Spudich -- Alternating-laser excitation of single molecules / A.N. Kapanidis ... [et al.] -- Orientation and rotational motions of single molecules by polarized total internal reflection fluorescence microscopy / J.F. Beausang ... [et al.] -- Imaging gene expression in living cells at the single-molecule level / J. Xiao ... [et al.] --

Single-virus tracking in live cells / M.J. Rust ... [et al.] -- Ultrasensitive imaging in live cells using fluorescent quantum dots / S. Courty and M. Dahan -- Imaging real-time gene expression in living systems / A.L. Wells ... [et al.] -- Single-molecule imaging of stochastic signaling events in living cells / S. Matsuoka ... [et al.] -- Fluorescence correlation spectroscopy in vitro and in vivo / E. Haustein and P. Schwille -- Optical traps to study properties of molecular motors / J.A. Spudich ... [et al.] -- High-resolution dual-trap optical tweezers with differential detection / C. Bustamante, Y.R. Chemla, and J.R. Moffitt -- Imaging and nanomanipulation of an actomyosin motor / S. Nishikawa ... [et al.] --

Single-molecule studies using magnetic traps / T. Lionnet ... [et al.] -- Probing polysaccharide and protein mechanics by atomic force microscopy / M. Rabbi and P.E. Marszalek -- Single-molecule analysis of nucleic acids and DNA-protein interactions using nanopores / M. Wanunu and A. Meller -- Single-molecule gold-nanoparticle tracking with high temporal and spatial resolution and without an applied load / A.R. Dunn and J. A. Spudich -- Advances in surface-based assays for single molecules / P.M. Fordyce, M.T. Valentine, and S.M. Block -- Hydrodynamic flow-stretching assay for single-molecule studies of nucleic acid-protein interactions / C.M. Schroeder ... [et al.].