Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts
Title:
Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts
Author:
Han, Dan
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (438 seconds)
Series:
Cancer Research
General Note:
Title from resource description page
Abstract:
We describe a detailed protocol for the isolation of tumor-initiating cells from human sarcoma patient-derived xenografts by fluorescence-activated cell sorting, using human leukocyte antigen-1 (HLA-1) as a negative marker, and for the further validation and characterization of these HLA-1-negative tumor-initiating cells.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/57011