Front Cover -- The Chlamydomonas Sourcebook: A Comprehensive Guide to Biology and Laboratory Use -- Copyright Page -- Table of Contents -- Dedication -- Preface -- Acknowledgments -- Chapter 1. An Overview of the Genus Chlamydomonas -- Introduction -- Description of the Genus -- Chlamydomonas Genetics: 1830-1960 -- Origins of the Major Laboratory Strains of Chlamydomonas -- Other Chlamydomonas Species Used Experimentally -- Chapter 2. Culture and Storage Methods -- Introduction -- Culture Media for Chlamydomonas reinhardtii -- Growing Chlamydomonas in Liquid Media -- Cultures on Agar -- Isolation of Chlamydomonas Species from Nature -- Mailing Chlamydomonas Cultures -- Storage of Chlamydomonas Strains -- Chapter 3. Cell Architecture and Division -- Introduction -- Early Studies of Cell Architecture -- The Cell Wall -- The Nucleus -- Mitochondria -- Basal Bodies, Flagellar Roots, and Cellular Microtubules -- The Eyespot -- Vacuoles, Microbodies, and Other Cellular Inclusions -- Mitosis -- Meiosis -- The Debate over Chromosome Number -- Mutational Disruption of Cell Structure and Division -- The Cell Cycle in Synchronous Cultures -- Chapter 4. The Sexual Cycle -- Introduction -- Types of Sexual Reproduction within the Genus Chlamydomonas -- Designation of Mating Types in Heterothallic Species -- Sexual Incompatibility within the C. moewusii Group -- Stages in the Reproductive Process -- Perturbations of the Mating Process -- Experimental Techniques for Mating Chlamydomonas -- Tests for Mating Type -- Quantitative Measurements of Mating -- Vegetative Diploids -- Chapter 5. Motility -- Introduction -- Flagellar Structure and Organization -- Flagellar Assembly -- Mutations Affecting Flagellar Structure and Function -- The Mechanism of Flagellar Motion -- Gliding Movement and Flagellar Surface Motility -- Cell Behavior -- Chapter 6. Metabolism.

Introduction -- Acetate Flagellates -- Starch Degradation -- Respiration -- Nonspecific Phosphatases -- Nitrogen Assimilation -- Sulfur Metabolism -- Vitamins and Cofactors -- Physiological Ecology -- Excretion of Metabolic Products into the Medium -- Why So Few Auxotrophs? -- Effects of Herbicides and Metabolic Inhibitors on Chlamydomonas -- Chapter 7. Photosynthesis -- Introduction -- Photosynthesis and Chloroplast Structure -- Chlorophyll Synthesis -- Carotenoid Pigments -- Other Pigment-Deficient Mutants -- Chloroplast Development in the y-1 Mutant -- Chapter 8. Organelle Heredity -- Introduction -- Inheritance of Chloroplast Genes in Crosses -- Mutations That Alter Transmission of Chloroplast Genes -- Genetic Mapping of Chloroplast Genes -- Uniparental and Biparental Inheritance of Chloroplast Genes in Other Chlamydomonas Species -- Chloroplast Genome Structure -- Transcription and Translation of Chloroplast Genes -- The Mitochondrial Genome -- The Role of Mating Type in Organelle Gene Transmission -- Chapter 9. Nucleic Acids and Protein Synthesis -- Introduction -- Chlamydomonas DNA: Characterization and Synthesis -- Enzymes Involved in DNA Replication and Transcription -- DNA Damage and Repair -- Ribosomes and Protein Synthesis -- Transcriptional and Translational Controls -- Chapter 10. Genetic Analysis -- Introduction -- Techniques of Mutagenesis -- Recovery of Mutants after Mutagenesis -- Analysis of Mutations -- Transformation -- Chapter 11. Mutant Strains -- Introduction -- A Consistent Nomenclature for Chlamydomonas Mutants? -- The Nuclear Gene Map of C. reinhardtii -- Strategies for Mapping New Mutations -- Nuclear Mutations Described in C. reinhardtii -- Mutations in Chloroplast Genes -- Mutants of C. moewusii and C. eugametos -- Mutant Strains of Other Species in Major Culture Collections -- Chapter 12. Procedures and Resources.

Introduction -- Preparation of Culture Media -- Histological Techniques for Chlamydomonas -- Fluorescence-Activated Cell Sorting -- Cell Fractionation Techniques -- Preparation of Chloroplast Fractions -- Assays for Photosynthesis and Other Metabolic Functions -- Preparation of Nucleic Acids -- Techniques for Studying Protein Synthesis -- Sources of Chlamydomonas Strains and Demonstration Materials -- Student Laboratory Exercises Using Chlamydomonas -- Doctoral Dissertations Relating to Chlamydomonas -- Bibliography -- Index to Algal Species -- Subject Index.