A Quantitative Glycomics and Proteomics Combined Purification Strategy
Title:
A Quantitative Glycomics and Proteomics Combined Purification Strategy
Author:
Hecht, Elizabeth S.
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (698 seconds)
Series:
Chemistry
General Note:
Title from resource description page
Abstract:
A high-throughput protocol was developed for combined proteomics and glycomics purification and LC-MS/MS quantification in plasma. Deamidation analysis of N-linked glycosylation motifs was specific to deglycosylated sites. Accurate quantitation of N-glycans was achieved by coupling filter aided N-glycan separation to the individuality normalization when labeling with glycan hydrazide tags strategy.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/53735