Isolation of Stem-like Cells from 3-Dimensional Spheroid Cultures
Title:
Isolation of Stem-like Cells from 3-Dimensional Spheroid Cultures
Author:
Hu, Wen-Yang
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (546 seconds)
Series:
Cancer Research
General Note:
Title from resource description page
Abstract:
Using human primary prostate epithelial cells, we report a novel biomarker-free method of functional characterization of stem-like cells by a spheroid-based label-retention assay. A step-by-step protocol is described for BrdU, CFSE, or Far Red 2D cell labeling; three-dimensional spheroid formation; label-retaining stem-like cell identification by immunocytochemistry; and isolation by FACS.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/60357