Key Concepts for PCR:<{dollar}> M. Innis and D. Gelfand,<{dollar}> Optimization of PCR: Conversations between Michael and David. J.J. Sninsky,<{dollar}> The Convergence of PCR, Computers, and the Human Genome Project: Past, Present and Future. R.D. Abramson,<{dollar}> Thermostable DNA Polymerases: An Update. B.R. Bloom,<{dollar}> Musings on Microbial Genomes. E.M. Beasley, R.M. Myers, D.R. Cox, and L.C. Lazzeroni,<{dollar}> Statistical Refinement of Primer Design Parameters. G. Zangenberg, R. Saiki,<{dollar}> Multiplex PCR: Optimization Guidelines. R. Wagner and A.D. Dean,<{dollar}> The Use of Immobilized Mismatch Binding Protein for the Optimization of PCR Fidelity. M.A. Northrup, L.A. Christel, W.A. McMillan, K. Petersen, F. Pourhamadi, L. Western, S. Young,<{dollar}> A New Generation of PCR Instruments and Nucleic Acid Concentration Systems. J.M. Kelley and J. Quackenbush,<{dollar}> Sequencing PCR Products. T.W. Myers,<{dollar}> Recent Advances in High-Temperature Reverse Transcription and PCR. S. Kaye,<{dollar}> Viral Genotyping by a Quantitative Point Mutation Assay: Application to HIV-1 Drug Resistance. J.R. Hully, In Situ<{dollar}> PCR. Quantitative PCR:<{dollar}> D.B. Bubois, C.R. WalkerPeach, M.M. Winkler, and B.L. Pasloske,<{dollar}> Standards for PCR Assays. C.T. Wittwer and M.G. Herrmann,<{dollar}> Rapid Thermal Cycling and PCR Kinetics. A.L.Hayward, P.J. Oefner, D.B. Kainer, C.A. Hinojos, and P.A. Doris,<{dollar}> Kinetics of Competitive Reverse Transcriptase-PCR. R. Higuchi and R. Watson,<{dollar}> Kinetic PCR Analysis Using a CCD Camera and without Using Oligonucleotide Probes. S.-Y.P. Chang,<{dollar}> Quantification of Telomerase Activity Using Telomeric Repeat Amplification Protocol. Gene Discovery:<{dollar}> K. Giese, H. Xin, J.C. Stephans, and X. Duan,<{dollar}> Differential Display. P.S. Nelson,<{dollar}> Single-Cell cDNA Libraries. J. Snider,<{dollar}> Whole Cell Assays. F. Mathieu-Daude, N. Benson, F. Kullmann, R. Honeycutt, M. McClelland, and J. Welsh,<{dollar}> Screening Differentially Displayed PCR Products by Single-Strand Conformation Polymorphism Gels. Y. Oh and L. Mao,<{dollar}> Microsatellite Protocols. P.M. Williams and A.L. Tucker,<{dollar}> Real-Time Quantitative PCR: Uses in Discovery Research. A.-L. Reysenbach and C. Vetriani,<{dollar}> Homology Cloning: A Molecular Taxonomy of the Archaea. F. Randazzo,<{dollar}> Cloning Mammalian Homologs of Drosophila<{dollar}> Genes. T.Seeley,<{dollar}> Cloning Human Homologs of Yeast Genes. Genomics and Expression Profiling:<{dollar}> J.J. Kang and M.J. Holland, <{dollar}> Cellular Transcriptome Analysis Using a Kinetic PCR Assay. M. Schena and R.W. Davis,<{dollar}> Parallel Analysis with Biological Chips. A.O. Schmitt, R. Herwig, S. Meier-Ewert, and H. Lehrach,<{dollar}> High Density cDNA Grids for Hybridization Fingerprinting Experiments. K. Chin and J.W. Gray,<{dollar}> Comparative Genomic Hybridization. T. Ferea, B. Dunn, D. Botstein, and P. Brown,<{dollar}> Genetic Footprinting and Fucntional Maps of the Yeast Genome. N.L. Simone, J.Y. Lee, M. Huckabee, K.A. Cole, R.F. Chaqui, C. Seshandri, B. Bonner, L.A. Liotta, and M.R. Emmert-Buck,<{dollar}> Molecular Analysis of Microdissected Tissue: Laser Capture Microdissection. T. Pastinen, A.-C. Syävnen, C. Moberg, G. Sitbon, and J. öLnngren, <{dollar}> A Fluorescent, Multiplex Solid-Phase MiniSequencing Method for Genotyping Cytochrome P450 Genes. M.A.D. Brow,<{dollar}> The Cleavase Enzyme for Mutation and Polymorphism Scanning. Index.

Pt. 1. Key concepts for PCR-- Ch. 1. Optimization of PCR: conversations between Michael and David-- Ch. 2. The convergence of PCR, computers, and the human genome project: past, present, and future-- Ch. 3. Thermostable DNA polymerases: an update-- Ch. 4. Musings on microbial genomes-- Ch. 5. Statistical refinement of primer design parameters-- Ch. 6. Multiplex PCR: optimization guidelines-- Ch. 7. The use of immobilized mismatch binding protein for the optimization of PCR fidelity

Ch. 8. A new generation of PCR instruments and nucleic acid concentration systems-- Ch. 9. Sequencing PCR products-- Ch. 10. Recent advances in high-temperature reverse transcription and PCR-- Ch. 11. Viral genotyping by a quantitative point mutation assay: application to HIV-1 drug resistance-- Ch. 12. In situ PCR-- Pt. 2. Quantitative PCR-- Ch. 13. Standards for PCR assays-- Ch. 14. Rapid thermal cycling and PCR kinetics-- Ch. 15. Kinetics of competitive reverse transcriptase-PCR

Ch. 16. Kinetic PCR analysis using a CCD camera and without using oligonucleotide probes-- Ch. 17. Quantification of telomerase activity using telomeric repeat amplification protocol-- Pt. 3. Gene discovery-- Ch. 18. Differential display-- Ch. 19. Single-cell cDNA libraries-- Ch. 20. Whole cell assays-- Ch. 21. Screening differentially displayed PCR products by single-strand conformation polymorphism gels-- Ch. 22. Microsatellite protocols-- Ch. 23. Real-time quantitative PCR: uses in discovery research

Ch. 24. Homology cloning: a molecular taxonomy of the archaea-- Ch. 25. Cloning mammalian homologs of drosophila genes-- Ch. 26. Cloning human homologs of yeast genes-- Pt. 4. Genomics and expression profiling-- Ch. 27. Cellular transcriptome analysis using a kinetic PCR assay-- Ch. 28. Parallel analysis with biological chips-- Ch. 29. High-density cDNA grids for hybridization fingerprinting experiments-- Ch. 30. Comparative genomics hybridization-- Ch. 31. Genetic footprinting and functional maps of the yeast genome

Ch. 32. Molecular analysis of microdissected tissue: laser capture microdissection-- Ch. 33. Amplified fragmant length polymorphism: studies on plant development-- Ch. 34. A florescent, multiplex solid-phase minisequencing method for genotyping cytochrome P450 genes-- Ch. 35. The Cleavase I enzyme for mutation and polymorphism scanning.