A Universal Protocol for Large-scale gRNA Library Production from any DNA Source
Title:
A Universal Protocol for Large-scale gRNA Library Production from any DNA Source
Author:
Köferle, Anna
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (632 seconds)
Series:
Genetics
General Note:
Title from resource description page
Abstract:
Methods for generating large-scale gRNA libraries should be simple, efficient and cost-effective. We describe a protocol for the production of gRNA libraries based on enzymatic digestion of target DNA. This method, CORALINA (comprehensive gRNA library generation through controlled nuclease activity) presents an alternative to costly custom oligonucleotide synthesis.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/56264