Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches
Title:
Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches
Author:
Kao, Shao-Yen
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (513 seconds)
Series:
Genetics
General Note:
Title from resource description page
Abstract:
Drosophila flight muscle is a powerful model to study transcriptional regulation, alternative splicing, metabolism, and mechanobiology. We present a protocol for dissection of fluorescent-labeled flight muscle from live pupae to generate highly enriched samples ideal for proteomics and deep-sequencing. These samples can offer important mechanistic insights into diverse aspects of muscle development.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/60309