Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids
Title:
Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids
Author:
Kim, Yubin
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (523 seconds)
Series:
Cancer Research
General Note:
Title from resource description page
Abstract:
This protocol provides detailed experimental steps to establish a three-dimensional in vitro culture of bladder tumor organoids derived from carcinogen-induced murine bladder cancer. Culture methods including passaging, genetic engineering, and orthotopic transplantation of tumor organoids are described.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/60469