High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping
Title:
High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping
Author:
Kosuta, Ceres
Personal Author:
Publication Information:
Cambridge, MA MyJoVE Corp 2016
Physical Description:
online resource (612 seconds)
Series:
Genetics
General Note:
Title from resource description page
Abstract:
Zebrafish have been used as reliable genetic model organisms in biomedical research, especially with the advent of gene-editing technologies. When larval phenotypes are expected, DNA extraction and genotype identification can be challenging. Here, we describe an efficient genotyping procedure for zebrafish larvae, by tail clipping, as early as 72-h post-fertilization.
Reading Level:
For undergraduate, graduate, and professional students
Subject Term:
Electronic Access:
https://www.jove.com/t/58024